ABSTRACT
ObjectiveThe study of the treatment of hypertriglyceridemia with scutellaria baicalensis stem-leaf total flavonoid (SSTF) are rarely reported.The objective of this study is to investigate the effects of SSTF on lipid metabolism and oxidative stress in rats with hypertriglyceridemia.Methods60 SD rats were randomly divided into normal group, model group, fenofibrate group (100 mg·kg-1), SSTF groups with low, medium and high doses (50,100,200 mg·kg-1, respectively). All rats, except those of the normal group, were fed with high-fat diet and given corresponding drug intervention for 6 weeks. Then the body masses at the 2nd, 4th and 6th weeks as well as wet weight of the liver at the end of 6th week were recorded, and liver index was calculated. The serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were detected. The levels of TG, TC, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in liver tissue were determined, and liver histopathological changes were observed by hematoxylin-eosin (HE) staining.ResultsIn the model group, compared with the normal group, the body masses at the 2nd, 4th and 6th weeks and liver index at the end of 6th week were increased, the serum levels of TG, TC and LDL-C got increased with decreased HDL-C level, and the levels of TG[（1.603±0.146）mmol/L], TC[（3.474±0.356）mmol/L] and MDA[（10.288±1.979）nmol/mg] in liver tissue were increased with decreased levels of SOD[（106.840±24.014）U/mg] and GSH-PX[（9.278±2.079）U/mg]. Compared with the model group,the fenofibrate group and all SSTF groups showed decreased body masses at the 2nd, 4th and 6th weeks and liver index at the end of 6th week, decreased serum levels of TG, TC and LDL-C with increased level of HDL-C, and decreased levels of TG, TC and MDA with increased levels of SOD and GSH-PX in liver tissue. The comparsion between the fenofibrate group and the high-dose SSTF group revealed that the body masses at the 4th and 6th weeks and liver index at the end of 6th week were decreased, the serum levels of TG, TC and LDL-C were decreased with increased level of HDL-C, and the levels of TG[（0.718±0.135）mmol/L] and MDA[（5.071±1.305）nmol/mg] in liver tissue got decreased with increased levels of SOD[（172.210±30.214）U/mg] and GSH-PX[（14.623±2.418）U/mg] in the latter group. All the above-mentioned differences were statistically significant (all P<0.05).ConclusionSSTF can regulate lipid metabolism and improve pathological injury of liver in hypertriglyceridemia rats, which may be related with inhibition of lipid peroxidation and reduction of oxidative stress.
ABSTRACT
Objective :To explore levels of cystatin C (CysC) ,N terminal pro brain natriuretic peptide (NT-proBNP) and cardiac troponin T (cTnT) in patients with acute heart failure (AHF) and diagnostic value of their combined de-tection .Methods :A total of 61 AHF patients treated in our hospital from Feb 2014 to Feb 2016 were selected ,in-cluding 21 cases of NYHA class II ,20 cases of class III and 20 cases of class IV .Another 61 healthy subjects under-going physical examination were treated as healthy control group during the same period .Plasma levels of CysC , NT-proBNP and cTnT were measured and compared between two groups and among different cardiac function class group .Predictive value of single and combined detection of CysC ,NT-proBNP and cTnT for AHF and its prognosis were compared .Results : Compared with healthy control group ,there were significant rise in plasma levels of CysC [(0.89 ± 0.12) mg/L vs.(1.64 ± 0.61) mg/L] ,NT-proBNP [(186.24 ± 15.35) ng/L vs.(2562.44 ± 69.67) ng/L] and cTnT [ (0.08 ± 0.04) μg/L vs.(0.32 ± 0.31) μg/L] in AHF group ,P＝0.001 all.Compared with NYHA class II group ,there were significant rise in plasma levels of CysC [ (1.23 ± 0.47) mg/L vs.(1.73 ± 0.58) mg/L vs.(2.11 ± 0.62) mg/L] ,NT-proBNP [ (867.45 ± 48.91) ng/L vs.(1868.24 ± 79.70) ng/L vs.(3786.56 ± 89.94) ng/L] and cTnT [ (0.25 ± 0.08) μg/L vs .(0.39 ± 0.24) μg/L vs.(0.49 ± 0.37) μg/L] in class III group and class IV group ,and plasma NT-proBNP level of class IV group was significantly higher than that of class III group ,P＜0.05 or ＜0.01. Compared with single detection of CysC ,NT-proBNP and cTnT ,there were significant rise in predictive positive rates of AHF (49.18%,50.82%,54.10% vs.90.16%) ,malignant arrhythmia (44.00%, 48.00%,48.00% vs .80.00%) and heart failure aggravation (50.00%,44.44%,38.89% vs.83.33%) of triple detection ,P＜0.05 or ＜0.01. Conclusion :Combined detection of CysC ,NT-proBNP and cTnT possesses high eval-uation and predictive value for AHF risk and prognosis ,which is worth extending .